| Cat# | Product Name | Price |
|---|---|---|
| Kit-2471 | Alkaline Phosphatase Assay Kit | Inquiry |
| Kit-0078 | Alkaline Phosphatase Colorimetric Assay Kit | Inquiry |
| Kit-0079 | Alkaline Phosphatase Assay Kit | Inquiry |
| Kit-0080 | Alkaline Phosphatase Fluorometric Assay Kit | Inquiry |
| Kit-0865 | Alkaline Phosphatase Fluorometric Assay Kit (Near Infrared) | Inquiry |
| Kit-2285 | Alkaline Phosphatase Activity Colorimetric Assay Kit | Inquiry |
Alkaline Phosphatase is an important enzyme molecule that is widely distributed in organisms. It is rich in expression in the bile, bones, liver, and other parts of the human body, and widely participates in the regulation of many cellular functions.
Alkaline Phosphatase is mainly used to detect enzyme activity in acidic environments. It is an endonuclease that can undergo cleavage within biological molecules, playing an important catalytic role. It plays a crucial role in human growth, bone and nervous system development. Therefore, the activity of Alkaline Phosphatase is of great significance for the judgment of health and disease.
At present, the common methods for detecting Alkaline Phosphatase activity in the human body are based on the principle of enzyme reactions. When Alkaline Phosphatase activity is present, the hydrolysis reaction of the substrate can be observed by adding a substrate, resulting in the formation of a yellow product. After reasonable color comparison, we can determine the content and activity of Alkaline Phosphatase based on the color difference generated by the sample.
The pNPP method is used to determine ALP activity through the enzymatic hydrolysis reaction of hydroquinone diphosphate (pNPP). The principle of this method is that pNPP is hydrolyzed into yellow phosphate under the catalysis of ALP, and its optical density can be measured by a spectrophotometer. This method is simple, easy to operate, with high accuracy and good sensitivity, and has been widely used in clinical testing.
The AS-5 rule is a method for determining ALP activity based on phosphate ion selective electrodes. Its principle is to add magnesium ions to the reaction system, causing ALP to catalyze the hydrolysis of substrate isopropyl phosphate (IAP) into orthophosphate, releasing phosphate ions, and causing electrical signals to appear on the electrode. ALP activity is measured by detecting the size of electrical signals. This method can avoid errors caused by different substrates and is also suitable for determining samples with acid phosphatase activity.
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