Aconitase is a type of iron sulfur protease widely present in cytoplasm and mitochondria, involved in energy metabolism and biosynthesis processes.

Aconitase participates in the reaction of pyruvate cycle and isocitric acid pathway in cells, and its main role is to catalyze the isomerization of isocitric acid and convert it into pyruvate. In addition, Aconitase is also involved in biological processes such as the biosynthesis of iron sulfur clusters and protein synthesis. It can be seen that Aconitase plays an important role in cellular energy metabolism.

The main methods for determining Aconitase activity include chromatography, electrophoresis, and spectrophotometry. Photometric method is an activity measurement method based on the colorimetric reaction generated by Aconitase. The sample is added to the reaction system containing substrate, and the isomerization of isocitric acid is catalyzed by Aconitase to produce detectable products. As the activity of Aconitase increases, the amount of reaction products generated increases accordingly. Finally, by measuring the colorimetric reaction generated by the Aconitase activity, the Aconitase activity can be obtained.

There are various Aconitase activity detection kits available on the market. The detection principle of these kits is roughly the same, and they all use NADPH to undergo colorimetric reactions to determine Aconitase activity. For example, one type Aconitase activity detection kit uses Aconitase to catalyze reactions in the test solution and generate pigments to determine Aconitase activity, and another Aconitise activity detection kit uses the substrate isocitric acid and pentachlorophenol in the reaction solution to generate detectable phenolphthalein pigment, and detects the activity of Aconitise by measuring the absorbance change.

SUBMIT