Product Overview :
The ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. This kit comes in a convenient 96-well format, with purified ACE2 and SARS-CoV-2 Spike proteins, HRP-labeled anti-mouse Fc region antibody, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of Fc-tagged Spike protein by HRP-labeled Anti-mouse-Fc. Only a few simple steps on a microtiter plate are required for the assay. First, ACE2 protein is attached to a nickel-coated 96-well plate. Next, SARS-CoV-2 Spike-Fc is incubated with ACE2 on the plate. Finally, the plate is treated with Antimouse-Fc-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.
Description :
Coronavirus disease 2019 (COVID-19) increases the risk of developing Acute Respiratory Distress Syndrome (ARDS), which is often fatal at the late stages of the infection when the SAR-CoV-2 virus causes significant damage to the lungs. As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.
Stability :
Up to 6 months from date of receipt, when stored as recommended.
Kit Components :
SARS-CoV-2 Spike Protein (RBD), mFc Tag, 5 µg, -80°C
ACE2, His-Tag, 5 µg, -80°C
3x Immuno Buffer 1, 50 ml, -20°C
Blocking Buffer 2, 50 ml, +4°C
Secondary HRP-labeled antibody 1 (mouse), 15 µl, -80°C
ELISA ECL substrate A (transparent bottle), 6 ml, Room Temp
ELISA ECL substrate B (brown bottle), 6 ml, Room Temp
Nickel-coated 96-well white microplate, 1, +4°C
Avoid multiple freeze/thaw cycles!
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