Description :
The 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit employs CFSE to label target cells within the mixed cell population and 7-AAD to label dead cells. This kit provides an improvement over the traditional 51chromium (51Cr) release assay to assess cell-mediated cytotoxicity. CFSE labeling is more sensitive, does not employ radioisotopes, and cytolysis can be assessed at the single-cell level. The mixed lymphocyte reaction is a cornerstone method of immunology1, and this kit can help immunologists to detect subtle changes in cytotoxic lymphocyte function. The kit provides sufficient reagents to effectively stain approximately 10^9 cells.
Kit Components :
7-AAD Viability Dye (1,000X), 2 vials/50 µl, 4°C
CFSE Stock Solution, 1 vial/100 µl, -20°C
Cell-Based Assay Buffer Tablet, 4 Tablets, RT
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