Human Alpha Fetoprotein Antigen Grade

• Cat.No.: CDA001

Specification

• Cat. No.: CDA001
• Description: AFP is a glycoprotein containing about 4% carbohydrate. AFP is produced by the fetal liver and is not normally expressed in adults.
• Source: Human fetal cord serum
• Format: Purified, Lyophilized. Reconstitute to 1mg/ml in 50mM Tris pH 7.5, 50mM ammonium bicarbonate pH 8.1 or PBS. Prior to reconstitution rap the bottom of the vial against a bench top to ensure all product is at the bottom of the vial. Allow the reconstitution buffer to equilibrate to room temperature before use. Allow the vial to sit without agitation for at least 5 minutes followed by vortexing at slow speed or gentle swirling.
• Contaminants: less than detectable limit of Albumin by SDS-PAGE.
• Purification: 80% pure (SDS-PAGE). Column chromatography
• Activity: 0.5mg/ml
• Buffer: Lyophilized from 1N PBS, pH 7.5
• Applications: Specific methodologies have not been tested using this product.
• Storage: Upon receipt, store at -20℃
• Warnings: All human source materials have tested negative for HIV1, HIV2, HCV antibodies and HbsAg. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.
• Tag: Non

Gene Information

• Gene Name: AFP
• Synonyms: Alpha-fetoprotein; Alpha-fetoglobulin; Alpha-1-fetoprotein; AFP; FETA; HPAFP; HP; OTTHUMP00000160480; FETA_HUMAN; Alpha-fetoprotein [Precursor].
• Gene ID: 174
• mRNA Refseq: NM_001134
• Protein Refseq: NP_001125
• MIM: 104150
• UniProt ID: P02771
• Chromosome Location: 4q11-q13
• Function: copper ion binding; metal ion binding; nickel ion binding

Reviews

11/14/2022

Investigating protein-protein interactions in cellular stress responses for cell survival.

02/15/2020

Dissecting protein-protein interactions in viral replication for antiviral strategies.

03/09/2017

Mapping protein-protein interactions in protein degradation pathways for proteostasis.

Q&As

Can AFP protein be utilized as a diagnostic or prognostic marker for certain diseases or conditions, and what experimental studies can be conducted to validate its clinical utility?

The clinical utility of AFP protein as a diagnostic or prognostic marker can be validated through clinical studies using patient samples, correlation analyses with disease outcomes, or comparison with existing diagnostic methods.

What are the downstream signaling pathways or cellular responses triggered by AFP protein binding or activation, and how can they be studied using molecular and cellular techniques?

Downstream signaling pathways or cellular responses triggered by AFP protein binding or activation can be studied using techniques such as Western blotting, gene expression analysis, or functional assays specific to the signaling pathways of interest.

Can AFP protein be targeted or manipulated to develop therapeutic interventions or treatment strategies, and what experimental approaches can be used to evaluate their efficacy and safety?

Therapeutic interventions or treatment strategies targeting AFP protein can be evaluated for efficacy and safety using in vitro or in vivo models, disease-specific assays, or clinical trials to assess their impact on disease progression, patient outcomes, or potential side effects.

What are the cellular and molecular mechanisms underlying the production and secretion of AFP protein, and how can they be experimentally elucidated?

The cellular and molecular mechanisms underlying the production and secretion of AFP protein can be elucidated through techniques such as gene expression analysis, protein trafficking studies, or immunofluorescence microscopy.

Are there any genetic variations or mutations in the AFP gene that impact its expression or function, and what are the implications for disease susceptibility or clinical outcomes?

Genetic variations or mutations in the AFP gene that impact its expression or function can be investigated through genetic association studies, functional characterization of mutant variants, or population-based analyses.

What are the regulatory factors or signaling pathways that control the expression and transcriptional regulation of AFP gene, and how can their involvement be studied?

The regulatory factors or signaling pathways that control the expression and transcriptional regulation of the AFP gene can be studied using techniques such as promoter analysis, transcription factor binding assays, or gene knockout/knockdown approaches.

What are the functional roles of AFP protein in embryonic development, tissue regeneration, or disease progression, and how can its biological functions be investigated experimentally?

The functional roles of AFP protein in embryonic development, tissue regeneration, or disease progression can be investigated through genetic or pharmacological perturbations, in vitro or in vivo model systems, or functional assays specific to the biological processes of interest.

Are there any specific biomarkers or imaging techniques that can be used to detect AFP protein in biological samples, and how can their sensitivity and specificity be assessed experimentally?

Specific biomarkers or imaging techniques for detecting AFP protein in biological samples can be developed and their sensitivity and specificity can be assessed using techniques such as ELISA, immunohistochemistry, or imaging modalities like MRI or PET.

How does AFP protein interact with its binding partners or ligands, and what experimental approaches can be used to study these interactions?

The interactions of AFP protein with its binding partners or ligands can be studied using techniques such as co-immunoprecipitation, surface plasmon resonance, or receptor-binding assays.

How does AFP protein contribute to immunomodulation or immune evasion, and what experimental strategies can be employed to investigate its immune-related functions?

The immune-related functions of AFP protein, such as immunomodulation or immune evasion, can be investigated using techniques like immune cell assays, cytokine profiling, or immune response assays.