Active Recombinant Human AGER protein(Met1-Ala344), hFc-tagged

• Cat.No.: AGER-3137H
• Product Overview: Recombinant Human AGER(NP_001127.1)(Met 1-Ala 344) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ala344
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human S100A12 at 2 μg/ml (100 μl/well) can bind recombinant human AGER with a linear range of 0.032-20 μg/ml.
• Molecular Mass: The recombinant human AGER/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 562 amino acids and predictes a molecular mass of 61.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhAGER/Fc monomer is approximately 80-90 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 70 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: AGER advanced glycosylation end product-specific receptor [ Homo sapiens ]
• Official Symbol: AGER
• Synonyms: AGER; advanced glycosylation end product-specific receptor; RAGE; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta
• Gene ID: 177
• mRNA Refseq: NM_001136
• Protein Refseq: NP_001127
• MIM: 600214
• UniProt ID: Q15109