Recombinant Human Von Hippel-Lindau Tumor Suppressor

• Cat.No.: VHL-232H
• Product Overview: Recombinant Human VHL b -domain produced inE.Coliis a single, non-glycosylated polypeptide chain. The protein contains 174 amino acids and has a molecular mass of 19.2 kDa.

Specification

• Cat. No.: VHL-232H
• Product Overview: Recombinant Human VHL b -domain produced inE.Coliis a single, non-glycosylated polypeptide chain. The protein contains 174 amino acids and has a molecular mass of 19.2 kDa.
• Description: Von Hippel-Lindau disease (VHL) is a dominant inherited syndrome characterized by the predisposition to develop various kinds of benign and malignant tumors, including clear cell renal carcinomas, pheochromocytomas and hemangioblastomas of the central nervous system and retina. VHL syndrome is caused by germline mutation in the VHL tumor suppressor, and VHL tumors are associated with loss or mutation of the remaining wild-type allele. VHL has two domains: a roughly 100-residue NH2-terminal domain rich inb sheet (b-domain) and a smaller a-helical domain(a-domain), held together by two linkers and a polar interface. VHL protein is also involved in the degradation of hypoxia-inducible factor (HIF).
• Source: Escherichia Coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGPEESGPEELGA EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRGHLWLFR DAGTHDGLLV NQTELFVPSL NVDGQPIFAN ITLP.
• Physical Appearance: Sterile filtered clorless solution.
• Formulation: The protein (1mg/ml) containing phosphate-buffered Saline (PBS) and 2mM EDTA, pH7.4
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.
• Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
• Tag: Non

Gene Information

• Gene Name: VHL von Hippel-Lindau tumor suppressor [ Homo sapiens ]
• Synonyms: VHL; HRCA1; RCA1; VHL1; HRCA1; RCA1; pVHL; Protein G7; VHL von Hippel-Lindau tumor suppressor ; Elongin binding protein; Von Hippel-Lindau syndrome;Von Hippel-Lindau tumor suppressor
• Gene ID: 7428
• mRNA Refseq: NM_000551
• Protein Refseq: NP_000542
• MIM: 608537
• UniProt ID: P40337
• Chromosome Location: 3p26-p25
• Pathway: Pathways in cancer; Renal cell carcinoma; Ubiquitin mediated proteolysis
• Function: Transcription factor binding