Protein Extraction Services

Protein extraction is key in both research and industry, crucial for understanding protein structures, functions, and interactions. It’s essential for proteomics and drug discovery, needing precision to work with different samples and meet unique research goals.

Creative BioMart offers top-notch protein extraction services customized to your needs. With cutting-edge tech and know-how, we provide various methods to suit downstream analyses, delivering dependable, tailor-made solutions for both research and industrial uses.

Overview

What is Protein Extraction?

Protein extraction involves isolating and purifying proteins from various sources like cells, tissues, or biological samples. This is a cornerstone technique in life sciences because getting proteins in their pure form is crucial for studying how they work, interact, and their role in disease or drug development. High-quality protein extraction is like having the perfect ingredients for a scientific experiment, helping researchers understand complex biological systems and disease mechanisms.

There are several methods to extract proteins, each suited for specific needs. The main ones include solution extraction , ultrasonic disruption , enzymatic hydrolysis , and chemical reagent methods . For example, solution extraction works well for proteins in the cytoplasm. If you’re working with samples that have stubborn cell walls, ultrasonic disruption is often your best bet. Conversely, for membrane proteins, enzymatic hydrolysis might be more suitable. Picking the right extraction method really depends on both the sample type and the specific proteins you’re targeting.

Applications of Protein Extraction

1. Research Fields
   • Proteomics: By extracting proteins, scientists can conduct genome-wide studies to see how protein expression changes under various conditions.
   • Gene Expression Analysis: Extract specific proteins to confirm their roles in regulating gene expression.
   • Functional Studies: Investigate the functionality and activity of proteins to understand their roles in organisms’ mechanisms.
   • Disease Research: By extracting proteins tied to diseases, we dig deeper into how these illnesses function and uncover new targets for possible treatments.
2. Biopharmaceuticals

   For making biological medicines like vaccines, monoclonal antibodies, and enzyme products, protein extraction is vital. It’s critical for keeping protein purity and activity intact during production.

3. Food Processing

   Functional proteins are extracted to boost the nutritional value of food, like extracting proteins from plants or animals to replace meat or enhance food functionality.

4. Environmental Monitoring

   Protein extraction methods are used to detect how pollutants affect microorganisms or aquatic ecosystems. By analyzing changes in specific proteins, researchers can evaluate ecological health.

What We Offer?

Types of Samples We Handle

• Animal tissues: brain, liver, muscle, etc.
• Plant tissues: leaves, roots, stems, etc.
• Cell line: primary cell, suspension cell, adherent cell.
• Microorganisms: bacteria, fungi, etc.

Customizable Extraction Solutions

Creative BioMart provides tailored protein extraction services for different protein types. And we offer high-volume extraction to meet large-scale research demands and use techniques that work well with follow-up analyses like mass spectrometry and ELISA, ensuring full support for diverse experimental needs.

Extraction modes	Protocols	We deliver	Advantages
Ion-exchange Gel filtration Affinity (broad-spectrum) Hydrophobic interaction	Client-supplied protocols Published protocols Improved protocols developed by Creative BioMart Protocols tailored to the client's requirements	Purified protein sample Specified protein concentration Special formulation to prevent protein from degradation Custom aliquoting as specified by customer Certificate of analysis as specified by customer	Strong expertise in protein technologies Competitive timeline Open pricing policy Performance under strict confidentiality Close attention to client's needs and requirements Weekly project updates Continued technical support after project completion

Our Services on Protein Extraction

Organelle protein extraction

At Creative BioMart, we extract and purify proteins from cell organelles, which helps in understanding protein roles and interactions specific to organelles.

Nuclear protein extraction

Our team at Creative BioMart is skilled in isolating nuclear proteins, which is crucial for exploring gene regulation and how nuclear proteins function.

Membrane protein extraction

With our membrane protein extraction services, Creative BioMart isolates proteins tied to cell membranes, making membrane protein research more accessible.

Cytoplasmic protein extraction

Creative BioMart specializes in drawing out cytosolic proteins, ensuring top-notch isolation for studies on protein functions and interactions.

Analytical and Downstream Services

Service Procedure	Technical Methods
Upstream Services	Gene Cloning & Expression Optimization
	Protein Engineering Services
	Directed Evolution
	Protein Design and Engineering
	Protein Labeling
	Site-Specific and Custom Labeling
Analytical & Downstream Services	Expression & Purification	Protein Expression and Purification Services
		Membrane Proteins Expression and Purification
		Endotoxin Removal Service
		Renaturation of Recombinant Proteins from Inclusion Bodies
	Modification & Functional Assays	PEGylation Services
		Protein Ubiquitinylation Service
		Protein Interaction Service
		Protein Quantitation Service
		Protein Characterization
		Protein Sequence Analysis and Function Prediction
		Protein Kinase Assay
		Enzyme Activity Assay
Downstream Development	Discovery and Translational Service	Metabolism Assays
		Epigenetics-Based Assay Development
		Protein Pathway Profiling
		Epitope Mapping
	Biopharmaceutical Development	Protein drug development and validation
		Protein stability study
		Biologic drug preparation

Service Workflow

Instruments and Equipments

• 
  Ultraspeed Refrigerated Centrifuge
• 
  HPLC System
• 
  Ultrasonic Crusher
• 
  MS Spectrometers

Case Study

Fig. 1. SDS-PAGE results of crude, dialyzed, and purified proteins by DEAE-sepharose FF column (column 1) and final purified GGT by sephadex G-100 column (column 2). (Shi G, et al. J Agric Food Chem. 2024;)

Case 1: Ion-Exchange & Gel Filtration Extraction of γ-Glutamyl Transpeptidase

γ-Glutamyl transpeptidase (GGT) from Toona sinensis shoots was purified using ion-exchange and gel filtration methods. Composed of two peptides (35 and 50 kDa), GGT played a role in forming sulfur-containing volatiles (SCVs), enhancing flavors like cooked onion. Optimal activity occurred at pH 7.0 and 50°C, influenced by ions such as Ca2+ and Mg2+. GGT’s application could restore the sulfur aroma in thermally processed vegetables that lost activity during drying.

Fig. 2. Yield and purity of A-OP, UA-OP, and AUA-OP. (Tang L, et al. Ultrason Sonochem. 2024)

Case 2: Ultrasonic/Alkali Extraction of Okara Protein

Okara protein (OP) is a valuable plant-based protein. This study used an alternating ultrasonic/alkali treatment to extract okara protein (AUA-OP), enhancing its functional properties and stability in emulsions. AUA-OP purity exceeded 80%. Compared to traditional methods, AUA-OP maintained its chemical structure while showing increased fluorescence, surface hydrophobicity, and ζ-potential. The treatment resulted in looser, smaller protein particles and improved water/oil holding capacity, emulsifying activity index (EAI), and emulsion stability index (ESI). After 28 days, emulsions with AUA-OP displayed stable droplet sizes and low creaming, highlighting its potential for stable protein-based products.

Fig. 3. Extraction kinetics of pumpkin leaf proteins under the various ultrasound duration times and amplitudes. (Mijalković J, et al. Molecules. 2024)

Case 3: High-Intensity Ultrasound in Protein Extraction from Pumpkin Leaves

This study focuses on recovering valuable proteins from waste, such as pumpkin leaves, using high-intensity ultrasound. The technique boosts protein yield by up to 40%, significantly more than traditional methods. Ultrasound, especially at 40% amplitude, enhances the extraction, yielding a RuBisCO-rich protein with strong antioxidant properties. The process modifies the protein’s structure, increases hydrophobicity, and alters solubility in acidic conditions. However, it decreases emulsifying ability as sonication amplitude rises. By optimizing ultrasound settings, high-value protein extracts can be produced for use in food and supplements.

Fig. 4. Separation of a mixture of Azurin- Strep -GG-Pap and mScarlet- Strep , here without the Azo-tag, on the α-CD affinity column. (Mayrhofer P, et al. Nat Commun. 2024)

Case 4: Light-Controlled Affinity Chromatography with Azo-tag

Affinity chromatography is a go-to for quick protein purification. Introducing the Azo-tag, a short peptide that responds to light, this new approach improves the process. It includes p-(phenylazo)-L-phenylalanine (Pap), which switches forms with UV light. In its trans form, Pap strongly binds to α-cyclodextrin (α-CD). Proteins with this tag stick to an α-CD matrix in normal light or darkness but release quickly when exposed to 355 nm light. We showcase this technique, Excitography, to purify proteins like enzymes and antibodies effortlessly, without harmful chemicals or harsh conditions. Adding light control gives a new edge to this separation method.

Fig. 5. SDS-PAGE and immunoblots with crustacean-allergic patients’ sera were tested under both non-reducing and reducing conditions for proteins from four insect species. (Carriço-Sá B, et al. Food Chem. 2024

Case 5: Quick Protein Extraction and Allergy Assessment from Edible Insects

Edible insects are becoming popular as a sustainable protein source. However, they might pose allergy risks for some people. This study evaluated proteins from four EU-approved insects for reactivity with IgE from crustacean-allergic patients. Researchers used 16 extraction protocols on Tenebrio molitor, Alphitobius diaperinus, Acheta domesticus, and Locusta migratoria. The best method involved a simple 3.5-hour extraction with 100 mM Tris-HCl + 4% SDS buffer at 60°C for 2 hours. This approach effectively isolated proteins that reacted with IgE from allergic individuals, primarily due to enhanced epitope exposure.

Why Choose Us?

• Cutting-Edge Tech : Utilizing the latest in protein extraction technology.
• Expert Team : A group of seasoned scientists.
• Flexible Services : Solutions tailored to fit client needs.
• Quality Assurance : Rigorous controls ensure high purity and yield.

FAQs

• Q: Can you handle unconventional samples like ancient specimens or environmental samples?

  A: Absolutely! We tailor our protein extraction methods to fit different sample types, including complex ones like ancient remnants or environmental samples.

• Q: Do you have strategies for low-abundance protein extraction?

  A: Yes, we use specialized enrichment and separation techniques to significantly boost the extraction efficiency of low-abundance proteins.

• Q: Can you provide flexible service plans for various sample sizes?

  A: Of course! We offer protein extraction services from small samples to high-throughput demands and customize solutions for projects of any scale.

• Q: Will the extraction process alter the natural structure of the target protein?

  A: No, we select appropriate methods based on the target protein’s characteristics to ensure it retains its natural structure and biological activity.

Additional Links and Resources